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Comparing the glutathione-scaffold site among a variety of other members of the thioredoxin superfamily also provides an explanation for their disulfide substrate preferences, for example, why glutaredoxins prefer GSSR in contrast to thioredoxins lacking most of the relevant residues (Fig
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Following repeated subcutaneous dosing to steady state, drug exposure in epididymal white adipose tissue and quadriceps peaked at 1 hour and declined between 4 and 8 hours, with 1-MNA in those tissues moving inversely, so target engagement is real but brief within each dosing interval
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